Firat, S. Ak, and H. Bozkurt, "Distribution of bovine viral diarrhoea virus (BVDV) in the genital system tissues of cattle," Vet. Arhiv., vol. 72, pp. 235-248, 2002.
M. Van Vuuren, "Bovine viral diarrhea virus infection in livestock in Southern Africa. CAB reviews," Perspecfives in Agriculture, Veterinary Science, Nutrifion and Natural Resources, vol. 2005, pp. 4-12, 2005.
H. Houe, A. Lindberg, and V. Moennig, "Test strategies in bovine viral diarrhea virus control and eradication campaigns in Europe," J. Vet. Diagn. Invest., vol. 18, pp. 427-436, 2006.
M. Hilbe, H. Stalder, and E. Peterhans, "Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves," J. Vet. Diagn. Invest., vol. 19, pp. 28-34, 2007.
T. Wittum, D. Grotelueschen, and K. Brock, "Persistent bovine viral diarrhoea virus infection in US beef herds," Prev. Vet. Med., vol. 49, pp. 83-94, 2001.
L. A. Moczygemba, "Review of the relationship between persistent infection of cattle with bovine viral diarrhea virus and feedlot morbidity and gain," Bovine Pr., vol. 37, pp. 155-161, 2003.
L. Potgieter, "Bovine viral diarrhoea and mucosal disease," Infect Dis Livestock, vol. 2, pp. 946-969, 2004.
J. Kampa, K. Stahl, and L. Renstrom, "Evaluation of a commercial erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples," Acta Vet. Scand., vol. 49, pp. 1-7, 2007.
C. Luzzago, M. Frigerio, and F. Tolari, "Indirect immunohistochemistry on skin biopsy for the detection of persistently infected cattle with bovine viral diarrhoea virus in Italian dairy herds," New Microbiol., vol. 29, pp. 127-131, 2006.
T. Meiring, L. Prozesky, E. R. Du Preez, and D. J. Verwoerd, "The diagnosis and prevalence of persistent infection with bovine viral diarrhea virus in South African feedlot cattle," Onderstepoort J. Vet. Res., vol. 78, p. 323, 2011.
T. Cornish, A. Van Olphen, and J. Cavender, "Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus," J. Vet. Diagn. Invest., vol. 17, pp. 110-117, 2005.
S. Kuhne, C. Schroeder, and G. Holmquist, "Detection of bovine viral diarrhoea virus infected cattleâ€“testing tissue samples derived from ear tagging using an Erns capture ELISA," J. Vet. Med. B., vol. 52, pp. 272-277, 2005.
J. Ridpath, B. Hessman, and J. Neill, "Parameters of ear notch samples for BVDV testing: Stability, size requirements and viral load," presented at the Paper Presented at: Proc Am Assoc Bov Pract Conf., 2006.
M. Al-Khaliyfa, E. Abuelzein, and A. Gameel, "Identification of cattle persistently infected with BVDV by ear-notch testing in Saudi Arabia," Vet. Rec., vol. 167, pp. 660-661, 2010.
J. Kampa, Epidemiology of bovine viral diarrhoea virus and bovine herpesvirus type1 infections in dairy cattle herds. Uppsala: Sveriges lantbruksuniv, 2006, 2006.
A. Lindberg and S. Alenius, "Principles for eradication of bovine viral diarrhoea virus (BVDV) infections in cattle populations," Vet. Microbiol., vol. 64, pp. 197-222, 1999.
P. Roeder and J. Harkness, "BVD virus infection: Prospects for control," Vet. Rec., vol. 119, pp. 143-147, 1986.
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(2014). Identification of Cattle Persistently Infected With BVDV (PI) By Ear-Notch Testing In Southeast of Iran. Animal Review, 1(4): 65-68. DOI:
Bovine viral diarrhea virus (BVDV) infection can induce a variety of economically important clinical manifestations in cattle herds. One potential outcome is the creation of calves that are viremic but immunotolerant to the virus. These persistently infected (PI) calves are the result of in utero exposure to BVDV prior to the development of a competent fetal immune system. The aim of this study was to detect of cattle persistently infected with BVDV by ear-notch testing in southeast of Iran. Ear-notch skin samples, 3 mm in diameter, were collected from a total of 127 cattle from 6 randomly selected herds (calves aged under 12 months ), using pliers usually used for ear tagging and skin notch sampling, as described by the manufacturer of the Herd check ELISA kit (IDEXX) for the detection of BVDV antigen in PI cattle. Overall, 0.78 per cent of the animals examined, were positive for BVDV antigen.This study identifies the cattle persistently infected with BVDV by ear-notch testing in southeast of Iran for the first time.
This study identifies the cattle persistently infected with BVDV by ear-notch testing in southeast of Iran for the first time.
Studies on Seasonal Performance of Newly Developed Bivoltine Silkworm (Bombyx Mori L) Hybrids Tolerant To Bmnpv and Effect of Temperature on Disease Induction
N. Suresh Kumar, T. Yamamoto, H. K. Basavaraja, and R. K. Datta, "Studies on the effect of high temperature on F1 hybrids between polyvoltine and bivoltine silkworm races of Bombyx mori L," International Journal of Industrial Entomology, vol. 2, pp. 123–127, 2001.
H. Lakshmi and Chandrashekharaiah, "Identification of breeding resource material for the development of thermo-tolerant breeds of silkworm, Bombyx mori L," J. Exp. Zool., India, vol. 10, pp. 55 – 63, 2007.
N. A. R. Begum, H. K. Basavaraja, P. G. Joge, and A. K. Palit, "Evaluation and identification of promising bivoltine breeds in the silkworm, Bombyx mori L," International Journal of Industrial Entomology, vol. 16, pp. 15–20, 2008.
H. Aruga, N. Yoshitake, and M. Owada, "Factors affecting the infection per os in the in the cytoplasmic polyhedrosis of the silkworm, Bombyx mori L," J. Sericult. Sci., vol. 32, pp. 41-50, 1963.
O. Kempthorne, The design and analysis of experiments. New York: John Wiley & Sons, Inc, 1952.
Y. Mano, S. Nirmal Kumar, H. K. Basavaraja, N. Mal Reddy, and R. K. Datta, "A new method to select promising silkworm breeds/combinations," Indian Silk, vol. 31, p. 53, 1993.
S. Sato, "Adaptability in cocoon yield of useful hybrids in the silkworm, Bombyx mori L," JIDP, vol. 12, pp. 121-129, 1975.
J. Jaroonchi, "Batch selection of Thai polyvoltine silkworm races 15th and 15 ky," Bull. Thai. Seric. Res. Trg. Cent., vol. 2, pp. 56-58, 1972.
M. N. Narasimhanna, "Contribution to the genetics of silkworm," Ph.D. Thesis, University of Mysore, Mysore, 1976.
G. Subramanya, "Eco-genetic approach for determination of high yielding breeds of silkworm, Bombyx mori L," Ph.D. Thesis, University of Mysore, Mysore, 1985.
P. J. Raju, "Studies on the hybridization and synthesis of new breeds of silkworm, Bombyx mori L," Ph.D. Thesis, University of Mysore, Mysore, 1990.
V. G. Maribashetty, "Evolution of superior bivoltine races of silkworm Bombyx mori L. for tropics," Ph.D Thesis, University of Mysore, Mysore, 1991.
G. V. Kalpana, "Breeding of superior races of silkworm, Bombyx mori L. for tropical climates," Ph.D. Thesis, University of Mysore, Mysore, 1992.
H. K. Basavaraja, "Studies on the synthesis of productive bivoltine strains of Bombyx mori L. through hybridization, selection and genetic evaluation," Ph.D. Thesis, University of Mysore, Mysore, 1996.
R. Mahadevappa, "Evolution of superior bivoltine races for tropical conditions," Ph.D. Thesis, Bangalore University, Bangalore, India, 2006.
D. Guruswamy, "Breeding of promising races of silkworm Bombyx mori L. and identification of suitable hybrids for rainfed area in Karnataka," Ph.D Thesis, Bangalore University, Bangalore, India, 2006.
H. Lakshmi, M. Ramesh Babu, A. K. Saha, Chandrashekharaiah, and B. B. Bindroo, "Studies on the season wise evaluation of productive bivoltine silkworm (Bombyx Mori L) hybrids in tropical conditions," International Journal of Integrative Sciences, Innovation and Technology, vol. 1, pp. 20- 30, 2012.
P. Sudhakara Rao, R. Singh, G. V. Kalpana, V. Nishitha Naik, H. K. Basavaraja, and G. N. Ramaswamy, "Evaluation and identification of promising bivoltine hybrids of silkworm, Bombyx mori L. for tropics," Int. J. Indust. Entomol., vol. 3, pp. 31-35, 2001b.
M. Ramesh Babu, H. Lakshmi, J. Prasad, J. Seetha Ramulu, Chandrashekharaiah, and A. K. Goel, "Evaluation and selection of potential bivoltine parents for silkworm (Bombyx Mori L.) breeding," Indian J. Seric., vol. 44, pp. 82-91, 2005a.
Y. Abe and C. Ayuzawa, "Susceptibility of larval tissues in the silkworm, Bombyx mori L., to the nuclear polyhedrosis virus. I. Changes of the susceptibility to the virus of mid-gut epithelial cells in the silkworm larvae treated with low temperature," J. Sericult. Sci., vol. 43, pp. 200-205, 1974.
H. Aruga, "Mechanism of resistance to virus diseases in the silkworm, Bombyx mori. (II) On the relation between the nuclear polyhedrosis and the cytoplasmic polyhedrosis," J. Sericult. Sci., vol. 26, pp. 279-283, 1957.
M. Himeno, F. Matsubara, and K. Hayashiya, "The occult virus of nuclear polyhedrosis of the silkworm larvae," J. Invertebr. Pathol., vol. 17, p. 292, 1973.
Ishimori, "On the relationship between the occurrence of grasserie and the temperature limit and the route of infection in the silkworm, Bombyx mori L," J. Seric. Sci., vol. 20, pp. 51-52, 1951.
S. Suzuki and T. Hayashita, "Survival curve of viruses infected in the 5th instar," J.Seric. Sci., vol. 24, p. 206, 1955.
E. A. Steinhaus, Principles of insect pathology. London: McGraw Hill Books Co., Inc, 1949.
F. Matsubara, Y. Go, H. Mori, and H. Oogashira, "Changes in the resistance to the infection with NPV induced by low and high temperature treatment in the germ-free silkworm, Bombyx mori," Journal of Sericultural Science of Japan, vol. 53, pp. 538-542, 1984.
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(2014). Studies on Seasonal Performance of Newly Developed Bivoltine Silkworm (Bombyx Mori L) Hybrids Tolerant To Bmnpv and Effect of Temperature on Disease Induction. Animal Review, 1(4): 57-64. DOI:
Season and region specific studies of silkworm Bombyx mori L. are of greater importance in identifying and understanding the adaptability of silkworm genotypes, which are largely influenced by climatic factors. The performance of the newly developed hybrid combinations was evaluated for twelve economic traits during pre-monsoon, monsoon and post-monsoon seasons of the year to understand genotype and environment interactions and their stability under fluctuating tropical environmental conditions with Bombyx mori Nuclear Polyhedro Virus (BmNPV) inoculation @ 2x106 PIB’s/larva. The data was subjected to two way analysis of variance and season-wise relative merit of individual hybrids was computed following Multiple Trait Evaluation Index method. The index values indicated that the hybrids BNR9 x BNR10 and BNR9 x BNR4 with higher average cumulative EI values were ranked I and II and establishing their superiority over multiple traits. Further the larvae soon after fourth moult were kept at room temperature (25ºC), low temperature (5ºC) and high temperature (32ºC) without feed for 6 hours and then inoculated perorally with BmNPV. The newly ecdysed fifth instar larvae when exposed to low temperature of 5ºC and starved enhanced larval susceptibility to BmNPV while the hybrids BNR9 x BNR10 and BNR9 x BNR4 exhibited higher tolerance to BmNPV at 25ºC and moderate tolerance at 32ºC.
This study documents the evaluation of silkworm hybrids under BmNPV inoculated conditions during different seasons of the year. The methodology includes the estimation of the tolerance of the hybrids to BmNPV during various seasons influencing the physiological activities affecting their growth and development as well as the expression of economic characters.